Definition of Yeast One Two-Hybrid System

Molecular biology technique used to identify interacting proteins in a yeast model. A protein of interest (the bait) is fused to the DNA-binding domain of a yeast transcription factor (usually GAL4). (Usually) a cdna library is fused to the GAL4 activation domain. The library is then introduced into yeast cells carrying the bait construct. Functional interaction of the bait with fusion proteins from the library bring together the binding and activation domains of the transcription factor, allowing activation of a reporter gene. (syn: yeast two-hybrid system) (NCI/OSP)

Yeast One Two-Hybrid System is from the group

Expression Technologies for DNA and RNA

Other Names for Yeast One Two-Hybrid System

Yeast One/Two-Hybrid System
Two Hybrid
Yeast One Hybrid System
Yeast Two Hybrid System

Source

NCI Thesaurus License

Topics #Yeast One/Two-Hybrid System Two Hybrid Yeast One Hybrid System Yeast Two Hybrid System